PhD student at the Institute of Biochemistry and Molecular Biology

The UKB combines top performance in medicine and research with excellent teaching as one of the most powerful university hospitals in Germany. Annually, over half a million patients are treated on an outpatient and inpatient basis at the UKB. Around 3,500 people study medicine and dentistry here, and over 600 people are trained in health professions each year. With around 9,900 employees, the UKB is the third-largest employer in the Bonn/Rhine-Sieg region. The following part-time position (25,025 hours/week) is to be filled as soon as possible at the Institute of Biochemistry and Molecular Biology of the University Hospital Bonn: PhD student (m/f/d). The position is limited until December 31, 2030. Mitochondria fulfil various essential functions for the survival of eukaryotic cells. Their biogenesis and functions depend on the import of more than 1000 different proteins that are produced as precursors on cytosolic ribosomes. The translocase of the outer membrane (TOM complex) forms the general entry gate. Subsequently, specific protein translocases import these proteins into the mitochondrial subcompartments. We identified a plethora of interaction partners of mitochondrial protein translocases, linking them to protein complex assembly, organellar contact sites, energy metabolism and quality control. In this project, we will study the molecular mechanisms that coordinate protein import with inter-organellar contacts and metabolism. We use highly innovative techniques in biochemistry, molecular and cell biology. This includes robot based genetic screens combined with fluorescent imaging and protein-protein-interaction studies coupled with proteomic assays. We study the functionality and fate of proteins using protein stability, aggregation, disaggregation assays and analyse the state of post-translational modifications with ubiquitin-family proteins. Moreover, we reconstitute the import and assembly of mitochondrial proteins synthesized in cell-free systems into intact organelles in vitro. We offer a well-equipped workspace in an active international team. The University of Bonn provides an excellent scientific research environment. The position starts as soon as possible and is for a period of three years, with the possibility of an extension.

• Study the molecular mechanisms that coordinate protein import with inter-organellar contacts and metabolism
• Use highly innovative techniques in biochemistry, molecular and cell biology
• Conduct robot-based genetic screens, fluorescent imaging, protein-protein-interaction studies, and proteomic assays
• Analyze protein stability, aggregation, disaggregation, and post-translational modifications
• Reconstitute import and assembly of mitochondrial proteins in vitro

• Highly motivated, team-oriented graduate with a strong interest in protein biochemistry, molecular and cell biology
• Experience with biochemical, cell and molecular biology techniques or proteomics is advantageous
• Candidates should hold a diploma or a master's degree in Biology, Biochemistry, Molecular Medicine, Chemistry or a related subject